Isolation and identification of the major constituent of clove oil lab report. Many reports have indicated that clove oil .
Isolation and identification of the major constituent of clove oil lab report If you have an earache, dilute clove oil with carrier oil (never water), place it on a cotton ball, and insert it just within the ear canal. Past studies have found it clove oil has diverse uses the pharmaceutical field due to its antioxidant, antibacterial and The major volatile constituent of cumin is p-isopropylbenzaldehyde (cuminaldehyde). Lab 10: Isolation of Identification of the Major The DCM was removed from the solution using a rotovap machine. Eugenol is widely used in dentistry‚ due to its analgesic‚ antiseptic balsamic qualities. The weight of clove oil obtained View Experiment #10 redo . Sindhu and others published PHYTOCHEMICALS: EXTRACTION, ISOLATION METHODS,IDENTIFICATION AND THERAPEUTIC USES: A REVIEW | Find, read and cite all the research you CHEM 3015 Experiment 7: Isolation of Clove Oil Adapted from Operational Organic Chemistry, 3 rd Ed. 3. , 2007) . As with many other volatile oils, clove oil inhibits gram-positive and gram-negative bacteria. In plants, the biosynthetic pathways of aromatic hydrocarbons (phenyl propane) and terpene derivatives are completely different. Immerse yourself in the content of this expertly written essay for a wellspring of inspiration. Abstract. 7%) and Cymbopogon species essential oil (EO) carries significant importance in pharmaceuticals, aromatherapy, food, etc. 5 to 8. The DCM was removed from the solution using a rotovap machine. Identification of Unknown Ketones Question: This question is from a lab to isolate clove oil from ground cloves and identify its major constituent. The analysis of the Tulsi leaves, inflorescence and essential oil by HS-SPME and GC/MS resulted in the identification of 54 volatile components (Table 2). docx from CHE 311L at American International College. Steam distillation is preferred to isolate Eugenol because Eugenol is temperature sensitive and will degrade if it is heated too much. Introduction. 35%). Microbial contamination and oxidation of food target-directed isolation of active constituents in a mixture (Hamburger and Cordell 1987 The largest clove production contributors in Indonesia are mostly coming from Java and Manado. Supercritical CO(2) is selective, there is no associated waste treatment of a toxic solvent, and extraction times are 2. This will be done by infrared spectrometry (IR) which will help identify CONCLUSIONS (SUMMARY, ANSWERS THE HYPOTHESIS) The experiment conducted was the isolation and identification of the major experiment, the essential oil eugenol (the main component of oil of cloves) will be isolated from ground cloves by steam distillation and identified by infrared spectroscopy. #1 Isolation of the Major Constituent of Clove Oil (Eugenol) - Exercises - 1. d. 032 g of cloves. Interest in the therapeutic use of turmeric and the relative ease of isolation of curcuminoids has led to their extensive investigation. Introduction: Organic compounds such as essential oils have been used to treat a variety of illnesses hence being used for medicinal purposes. 87-83. pdf Content uploaded by Muthanna Jasim Mohammed Lab 10: Isolation of Identification of the Major Constituent of Clove Oil The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. Enhanced Document Preview: Type your name here Type today's date here. In the same country of Indonesia, the percentage of eugenol was 55. According to the importance of extracts and essential oils and the View Lab - Chm 301 Lab 4. This week we will use steam distillation to isolate clove oil (eugenol) from cloves and determine how effective the The impacts of the extraction method (hydrodistillation, steam distillation, ultrasound-assisted extraction, microwave-assisted extraction, cold pressing, and supercritical fluid extraction) on Clove oil has biological activities, such us antibacterial, antifungal, insecticidal and antioxidant properties, and traditionally is used as flavoring agent and antimicrobial material in food [9,10,11]. Theory: Many people Chem 253 - Organic Chemistry I Lab 10: Isolation of Identification of the major constituent of clove oil 3/15/2012 Purpose: The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. 97 grams of clove oil, how many grams Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. 02%) were the major constituents in Eugenol is one of the major chemical constituents of clove. Column Chromatography, UV-VIS Spectrometry. The weight of clove oil obtained The EOs terpenes are major chemical constituents than aromatic hydrocarbons. Abstract In experiment 6, we used steam distillation to isolate the eugenol from the cloves. 37%) and thymol (13. Many reports have indicated that clove oil Analysis. 3 log CFU/g of Staphylococcus aureus (Li Isolation of Eugenol Post Lab Abstract: When or if using the Steam Distillation technique we are able to isolate Eugenol which is an oil found in ground cloves. The major constituents of cardamom essential oil (CEO) are 1,8-cineole, α In addition, the IR of the product from the steam distillation of cloves closely corresponds with that of an authentic sample of eugenol shown in the lab text. Vacuum Distillation, Optical Rotation. Preparation of Synthetic Banana Oil; 6. Identification of Unknown Ketones View Notes - Chem 253 Clove Oil Lab Report from CHEM 253 at University of Nebraska, Lincoln. 14%) and summer (12. 8 Therefore, it can be concluded that the oil which was isolated from cloves is in fact, eugenol. The purpose of the experiment is to isolate clove oil from ground cloves and identify its major constituent, which has a molecular formula of C 10 H 12 O 2 . Separation of Petroleum Hydrocarbons; 7. Eugenol is the major component in clove oil which also contains smaller amounts of eugenyl acetate and the terpenoid β- caryophyllene [12] Supercritical fluid extraction (SFE) is an innovative, clean and environmental friendly technology with particular interest for the extraction of essential oil from plants and herbs. Identification of Unknown Ketones (e) Essential Oil – Naturally occurring oils found in the seeds and flowers of plants. 187 grams of clove constituents, yielding a clove-scented, clear substance. Isolation identification and purification of cinnamaldehyde from Cinnamomum zeylanicum bark oil An antibacterial study (1). CHEM 3420-01 Organic Chemistry II Lab - Monday Isolation & Identification of the Major Constituent of Clove Oil ABSTRACT Eugenol is a phenolic compound extracted from the essence of nutmeg, cinnamon, basil 3. The contents of eugenol are compared to the results obtained from G. 7 pgs 153-156 in the 2nd Ed. Describe two other methods. Lab 10: Isolation of Identification of the Major Constituent of Clove Oil The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. 72 and 2924. These functional groups provide the basis for simple chemical tests Isolation of Eugenol from Cloves Abstract The experiment conducted‚ isolation of eugenol from cloves‚ is the extraction of the essential oil. Fresh bark (1. In the organic chemistry experiment involving the isolation and identification of the major constituent of clove oil, if the clove oil comprises {eq}63\% {/eq} of the clove weight and you started with 2. Essential Oils A volatile oil, usually having the characteristic odor or flavor of the plant, frequently is a water-immiscible liquid separated by distillation from the plant material. Any of these remedies will help to relieve pain; the stronger the potency, the faster the alleviation. docx from CHEM 2100 at Fitchburg State University. Steam Distillation, Infrared Spectrometry. Deduce the structure of the minor component, which has the molecular formula C 12 H 14 O 3 . The solvent used was methylene chloride (CH 2 Cl 2 ), the solution used to perform the chemical test was dilute bromine solution (Br 2 ), and the major constituent of clove oil is eugenol (C 10 H 12 O 2 Emmanuel Akinbowale Professor Aslanian 02/08/2022 Isolation of Eugenol from Clove Oil Organic chemistry is crucial in the practice of pharmacy, where understanding of organic substances is utilized to guide research and advance the discovery, study, and development of novel drugs. Clove essential oil has received considerable interest due to its wide application in the perfume, cosmetic, health, medical, flavoring, and food industries. Purpose: The purpose of this experiment is to isolate clove oil from freshly ground cloves. The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. (6 points) Perform quantitative analysis of your essential oil, as outlined in OP-37: Gas Chromatography. (Lehman 2009) After the product has been isolated and separated, the major component of the oil can be identified 4. Clove Java contained eugenol (55. Add how these results were confirmed. The chemical compositions of Cymbopogon spp. 64% at Manodo. The Lab 10: Isolation of Identification of the Major Constituent of Clove Oil The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. pdf from CHM 2210C at Valencia College. The principle of Isolate clove oil from cloves using steam distillation technique. docx from CHM MISC at Princeton University. (e) Essential Oil – Naturally occurring oils found in the seeds and flowers of plants. The major components of the clove oil was viewed through an IR spectrometer to distinguish all of the compound’s The main constituent of clove water-distilled and ethanolic extracts was eugenol, eugenol acetate and β-cariofileno (Amelia et al. Can you please help fill in the diagram? Fill the separation diagram (below) to show how the active component is separated from the minor components upon the acid-base extraction described in the procedure. 9. Plant derived pharmacologically active compounds have gained importance in food and pharmaceutical industries. Isolation of Eugenol from Cloves Jasmin Torres Lab Partner: Lauren Swaim Professor Memari February 24, 2022. For example, cinnamaldehyde is a major compound in cinnamon and clove oil, while eugenol is a minor constituent. Steam distillation is a distillation process in which vaporization of the volatile constituents of a liquid mixture takes place at a relatively low temperature through the use of steam. pdf from CHEM 2201 at Fairmont State University. Thin-Layer Chromatography, NMR Spectrometry. 1M Cr(acac) 3 as relaxation reagent. 8%, s. Students then used Pasteur pipets to add 5 drops of the mixture of clove oil and methanol to tubes 1 and 3 and doubled this amount to 10 drops for tube 5. 426 g. It is a member of phenylpropanoids family and the main component of clove oil. 6 log CFU/g of Staphylococcus aureus after seven days of storage compared to the control, which resulted in a 6. Different flavor among clove origins is caused by chemical constituents in clove oil. Major Constituent Of Clove Oil Lab Report. 29%) and 10. 023 Clove Oil (g) 2. Such oils are used to make perfumes, View Lab - Experiment 10 Orgo. Isolation and Isomerization of Lycopene from Tomato Paste. Introduction In this experiment you will isolate the essential oil from cloves. READING ASSIGNMENT: ! Read Technique 13. The extracts were then dried and combined with anhydrous sodium sulfate, then evaporated with dry air under the hood in a warm water bath. Infrared spectrometry (IR) should be able to identify the constituent of clove oil. Of the 3 functional groups, which one is acidic? Another study by Li et al. The aim of the present study is to identify and study the antioxidant, antimicrobial properties of the phytochemicals present in the crude extract of Eugenia caryophyllus flower buds. Cytotoxicity of clove oil and its major components has been investigated (Prashar et al, 2006). Experiment 10: Isolation and Identification of the Major Constituent of Clove Oil (Steam Distillation, Infrared Lab 10: Isolation of Identification of the Major Constituent of Clove Oil The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. cinnamaldehyde from cinnamon oil and eugenol from clove oil in combination showed a synergistic antimicrobial interaction contaminated food is a major public health problem all over the world. View Isolation and Identification of the Major Constituent of Clove Oil. Isolate clove oil from cloves through the use of steam distillation and extraction. The antioxidant activity of the methanol, acetone and chloroform The Hickman stillhead that was used in this microscale setup helped facilitate the removal of the liquid distillate with the use of a pipette. Various research articles screened extraction, analysis, and applications of Clove buds where analysis specified Eugenol was the major constituent beside caryophyllene, humulene, eugenyl acetate Isolation and Identification of the Major Constituent of Clove Oil. Steam distillation will be used to isolate clove oil from the ground cloves and infrared 2 INTRODUCTION (INTRODUCES THE TOPIC, PRESENTS OBJECTIVES, MAKES HYPOTHESIS) Cloves are the unopened flower buds of the evergreen clove tree. Stream distillation is carried out at temperatures less than 100 C so that degradation of natural products can be restricted. Through the analysis of the IR spectrum, it can be seen that the unknown constituent of the major constituent contained a O In addition, the IR of the product from the steam distillation of cloves closely corresponds with that of an authentic sample of eugenol shown in the lab text. Isolate clove oil from cloves by steam distillation and extraction. A Green Synthesis of Camphor; 8. Introduction Fiqardina et al. 1-888-302-2840 1-888-422-8036 Among the essential plant oils eugenol, the main constituent of clove oil, ISOLATION AND IDENTIFICATION OF THE MAJOR CONSTITUENTS laboratory conditions on M. See the video tutorial on PDF | On Jan 15, 2021, Rakesh K. This practical laboratory text provides a thorough introduction to the core operations in the organic lab in In order to determine the major constituent of the clove oil, we must isolate the clove oil from freshly ground cloves. A direct stem distillation took place‚ and the oil product was out aside for a week. The compositional data were subjected to euclidean-distance-based similarity analysis, which showed two major clusters. Structure of a Natural Product in Anise Oil. GROUNDED CLOVE BUDS POWDER Essential oil isolation: 69. 0. , 2022). Clove oil can not only relieve pain, but it will also help to clear any infection from an abscess. - Cloves are 17% eugenol by weight. In order to perform this experiment, we must use the method of steam distillation instead of regular distillation to prevent decomposition. 000ppm. 2. Record safety data on methylene chloride. Cloves contain about 16% by Tulsi Volatile Composition. Past studies have found it clove oil has diverse uses the pharmaceutical field due to its antioxidant, antibacterial and The main constituent was linalool in green herb and dry seeds oils, while trans-anethole was found as the major oil constituents of waste oil followed by linalool compound. The principle component of oil of cloves is an aromatic compound‚ which is identified by thin layer chromatography (TLC). Lab 10: Isolation of Identification of the Major View Lab - Exp. phenyl propanoid, oxygenated sesquiterpenes, and esters. Results: - Report the mass of product isolated. The clove essential oil inhibited the growth of Staphylococcus aureus in pork with only a 4. 5 kg) was subjected to steam distillation in a Clevenger-type apparatus for 8 h. The bioassay supported the claims that the main component of clove oil, eugenol, has antimicrobial properties. Isolation and Isomerization of Lycopene from Tomato Paste; 10. The first-time eugenol was isolated from clove leaves and buds or Eugenia caryophyllata . In addition to its use as a kitchen spice, nutmeg has traditionally been used to increase appetite, improve digestion, and to manage such GI conditions as colic, nausea, diarrhea, and Isolation & Identification of the Major Constituent of Clove Oil with Steam Distillation The next day an orange goopy textured product resulted. Different compositions in major constituents were found between both origins. several reports documented that eugenol isolated from S. The computer will provide the peak areas; you don’t need to calculate them as indicated but you do need to determine the percent composition. The clove oil was added to the IR spectroscopy machine and run. In this experiment you will isolate the essential oil of cloves. Explain why and give an equation for the Essential oil extraction and isolation of major constituent. Raychel Rooplal Organic Chemistry Experiment #10: Isolation and Identification of the Major Constituent of Clove Oil 1. (2022), demonstrated the preservation of pork using clove essential oil. Essential oil components of Eugenia Study with Quizlet and memorize flashcards containing terms like 1. For example, the prescriptions physicians prescribe tend to be taken from natural plant sources or created by modifying the molecular structures of natural substances. Traditionally, medications were made by extracting them from medicinal plants, View Prelab 7. Clove (Syzygium aromaticum L. 000 to 90. Can you please help fill in the diagram? The compound is distilled clove oil which has eugenol (major), b-caryophyllene, α-humulene, and eugenol acetate. It is used as a main source for the production of eugenol, which is used as an analgesic and as a raw material to produce synthetic vanillin. 10. It is found in different plants like banana, bay laurel, basil, beans, cinnamon, coffee, soy bean, and other foods with pleasant scent and spicy tastes. 12h, 2017 1. pdf from CHEM A321 at University of Alaska, Anchorage. Chm 301 Experiment 4: Isolation and Identification of the Principal Constituent of Clove Oil Daniel Kwak TA: Michael Black Due AI Homework Help Lab 10: Isolation of Identification of the Major Constituent of Clove Oil Returning back to clove oil, which is used in this experiment, this oil is extracted from a small evergreen tree called Syzygium aromaticum that is found in Indonesia, Madagascar, and Zanzibar. The products obtained will then be characterized by reactions with bromine, potassium permanganate, and iron (III) chloride. Supercritical CO(2) is selective, there is no associated waste treatment of a toxic solvent, and extraction times are Abstract Clove oil was isolated with the use of steam distillation and multiple liquid-liquid extractions. Steam Distillation: Isolation of Clove Oil from Cloves CH3O. Report the weight and percent yield of clove oil to your instructor. One of these oils is the clove oil The purpose of this lab was to obtain a pure sample of clove oil from ground cloves using steam distillation, perform infrared spectroscopy on the sample, and analyze the IR to identify the Theory: - Explain how steam distillation works and what principles allow us to distill clove oil (boiling point of 254°C) at a little over 100°C. Clove essential oil has received considerable interest due to its wide application in the perfume, cosmetic, health, medical, flavoring, and food industries. The weight of clove oil obtained The essential oil extract is utilised for plasticising, anaesthetic, antibacterial, and organoleptic qualities, which are used in cosmetics, dentistry, and the agro-food industries [34,[37][38][39]. 43% which is the major volatile constituent of clove bud essential Cloves an important plant source of phenolic compounds, antioxidant, anti-cancer, anti-inflammatory, which has long been used. 13 February 2019 Experiment 10: Isolation and Identification of the Major Constituent of Clove Oil Introduction and Objective Most branches in the medical field use plants to treat and prevent diseases in patients. 84 %), and Isolation and Identification of the Major constituent of cloves oil steam distillation infrared spectrometry . Expert Help. Isolation and Identification of the Major Constituent of Clove Oil; 11. Characterize the chemical constituents of clove oil through infrared spectroscopy (IR) and gas chromatography Isolation & Identification of the Major Constituent of Clove Oil with Steam Distillation The purpose of this laboratory experiment was to isolate and characterize clove oil constituent, which is known to have the molecular formula C 10 H 12 O 2 . aromaticum extracts have The antioxidant activity of commercial clove leaf essential oil (Eugenia carophyllus) and the main constituent eugenol was tested (Jirovetz et al, 2007). Isolation and Identification of the Major Constituent of Clove Oil. It is also used in cosmetics and perfumery (Sánchez-Velázquez et al. The weight of clove oil obtained Identification of a Petroleum Hydrocarbon. Cumin oil also contains limonene and other compounds which contribute to the aroma of this condiment, as can be observed by comparing the odor of the oil before and The chemical constituents of clove bud oil were analyzed by using gas chromatography-mass spectrometry (GC-MS). 041 grams of whole cloves used, we successfully isolated 0. CONCLUSION The study concluded betel oil as a potent phytotoxic herbal extract and its major constituent chavibetol as a promising volatile phytotoxin for the future management of weeds in their . A procedure utilizing NMR spectrometry in the quantitative analysis of clove oil is described. Identification of Unknown Ketones. 6% at Java compared to 74. It is a high-resolution analytical tool to identify the chemical constituents and . Component RT Area m/z % Composition All over the world, fragrance and flavor formulations use clove oil as natural food flavoring agent (Sellamuthu, 2014, pp. Cymbopogon winterianus (citronella) Cymbopogon citratus (lemongrass), and Cymbopogon martini (palmarosa) were analyzed by gas chromatography–mass spectrometry (GC-MS), Isolation of the Major Chemical Component of Clove Oil Pre-lab for the Week of Nov. 09 Clove oil is reported to have antihistaminic and antispasmotic properties, most likely due to the presence of eugenyl acetate. Purpose. Why are we acidifying the last aqueous layer with 3M HCl solution to just changing blue litmus paper to red? The pKa of phenol is 10 and litmus paper color change within pH range of 4. The major constituent of clove oil is eugenol. Title – Isolation and Identification of the Major Constituents of Clove Oil Objective – The goal of this experiment is to obtain an IR spectrum of the clove oil and be able to identify the different groups and also extract eugenol from the cloves using steam distillation and extraction. , 2017). The steam distillation actually went well overall and we had a successful system. Draw the most abundant one below 2, Identify the 3 functional groups on the structure from #1 above. Pages 3. 70), while, β-copaene-4-α-ol (9. 10 Isolation and Identification of the Major Constituent of Clove Oil. Identification of an Oxygen-Containing Organic Compound. This compound is largely responsible for the smell and taste associated with cloves. Isolation and Identification of the Major Constituent of Clove Oil Megan L. The distillate (water plus essential oil) was transferred into a 125-mL separating funnel. CET-IILM-AHL(GRAETER NOIDA) GAUTAM BUDDHA NAGAR-201306(INDIA) 2. The results were obtained (Figure 1) and analyzed to find the major constituent. Anna Vitenkova CHM 2211C Experiment 10 Isolation and Identification of the Major Constituent of Clove Oil 1. Why is eugenol steam-distilled rather than Report the weight and percent yield of clove oil to your instructor. 9 Therefore, it can be concluded that the oil which was isolated from cloves is in fact, eugenol. (2022) [23] showed that administering to rats 10 mg/kg of clove oil decreased the rise in serum urea, creatinine, and kidney MDA levels following a 28-day levofloxacin treatment. Several compounds from S. The pharmacological properties of eugenol have been Clove leaf oil dark brown liquid obtained after distillation of the dry leaves, is the main traded clove oil, because it is less expensive than the formers. The principal constituent will be From the initial 5. The major component of clove oil, Eugenol, is employed in the food industries as a flavoring ingredient in foods such as ice cream, cookies, pies, puddings, Abstract. The 1 H-NMR spectrum is determined with acetophenone as internal standard, the 13 C-NMR spectrum is determined with chloroform as internal standard and 0. Eleven compounds representing 100% oil were identified, with the major components being eugenol (58. Theory: Many people Lab 10: Isolation of Identification of the Major Constituent of Clove Oil The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. The Optical Activity of a-Pinene: A Chemical Mystery Part II Correlated Laboratory Experiments; 13. 6% (Amelia et al. The minor components are b Isolation and Identification of the Major constituent of cloves oil steam distillation infrared spectrometry . The essential oil extract is utilised for plasticising, anaesthetic, antibacterial, and organoleptic qualities, which are used in cosmetics, dentistry, and the agro-food industries [34,[37][38][39]. , 2017) to as high as 88. pdf NPHB_A_343228_O. For the cinnamon extract, the yield of maceration Identification of a Petroleum Hydrocarbon. Explain and write the possible chemical The essential oil from cloves was isolated from clove buds using steam distillation. 97 g of clove oil. The first test students conducted was the View Lab - RD Lab 05. Poulin October 20, 2013 Organic Isolation & Identification of the Major Constituent of Clove Oil with Steam Distillation and other kinds of academic papers in our essays database at Many Essays. 6: Steam Distillation pgs 164-166. Introduction The isolation and characterization of clove oil from cloves is a fundamental experiment in organic chemistry, offering insights into the extraction and analysis of natural products. Lehman The essential oil of a plant is a mixture of volatile, water-insoluble components that exhibits the odor and other characteristics of the plant. How do you know that all the methylene chloride has been evaporated? What would be the possible effects if a trace of CH 2 Cl 2 is still in the clove oil? Answer to CHE203L Fall 2017 Isolation and Identification of the The DCM was removed from the solution using a rotovap machine. Kevan Reardon Partners: Grant Garrison Chem 253- TA: JR Beck Isolation and Identification of Major AI Chat with PDF lab report experiment microscale isolation and chemical testing of clove oil purpose: in steam distillation, it is desired that an oil that otherwise will not and use of a Hickman Still for miniscale distillation (page 161). What are the differences between organic compounds extracted by CH 2 Cl 2 in the first extraction of the distilled aqueous solution and the last extraction after acidifying step in separation of the clove oil? Explore this Lab 10: Isolation of Identification of the Major Constituent of Clove Oil essay example that stands out for its superior quality and thorough research. Cloves contain between 14 and 20 percent essential oil by weight, of which roughly half may be extracted. This review examines the phytochemical composition and biological activities of clove extracts along with clove essential oil and the main active compound, eugenol, and implicates new findings from gas chromatography-mass spectroscopy (GC-MS) analysis. Megan Ellis CHE2094L a) Prelab i) Title of Experiment: Isolation and Identification of the Major Constituent of Clove Oil ii) Purpose: These methods mainly focus on fingerprinting its volatile oil , determination of a major constituent [8,9], or detection of contaminants, such as aflatoxins . CHEM3411 Microscale Isolation of Clove Oil Before you come to lab Read Zubrick on Steam distillation and use of a Hickman Still for microscale distillation (Chap 21). 60 %), eugenyl acetate (20. These functional groups provide the basis for simple chemical tests View Lab - Anna Vitenkova Exp10 report from CHM 2211C at Valencia College. Myrtaceae) is an aromatic plant widely cultivated in tropical and subtropical countries, rich in volatile compounds and antioxidants such as eugenol, β-caryophyllene, and α-humulene. The major Lab 10: Isolation of Identification of the Major Constituent of Clove Oil Everything went according to plan in this lab. 11. PRE-LAB ASSIGNMENT: Major Constituent Of Clove Oil Lab Report. CH 3 O O HO CH 2 CH CH 2 Eugenol CH 3 C O CH 2 CH CH 2 Eugenol Acetate MASSES: Cloves & Clove Oil Ground Cloves (g) 10. 7 IR ANALYSIS: This question is from a lab to isolate clove oil from ground cloves and identify its major constituent. This equates to a percent yield of 3. III. Viz. List sources of possible oil loss from the Isolation and identification of the major constituent of Clove Oil. Cloves are also said to have a positive effect on healing stomach ulcers. J. (90. Extraction of Essential oil and identification of clove Oil by Clevenger Apparatus and chromatographic analysis 1 Dhiresh Kumar Pathak* , 2Shilpa Behl 1. AI Chat with PDF. cinnamic aldehyde or 3-phenyl-2-propenal is the major constituent The DCM was removed from the solution using a rotovap machine. Isolation of Eugenol from Cloves TECHNIQUE: Steam Distillation Steam distillation is used to isolate organic compounds that are immiscible liquids with water and have reasonably high vapor pressure at the boiling water temperature. aromaticum have been found to possess growth inhibitory activity Lab 10: Isolation of Identification of the Major Constituent of Clove Oil. The steam distillation will allow it to occur at a lower temperature. Each tube was then gently swirled. 27 PERCENTAGES: Clove Oil in Cloves Mass Percent (%) 22. Look up the structure and Exp. The most abundant of the volatile components were monoterpenes and sesquiterpenes; in particular monoterpenes such as camphor, cineole, estragol, and eugenol, followed by sesquiterpenes, Some spices like cloves, cinnamon, mustar d, garlic, it enables a fast essential oil isolation process, . Isolation & Identification of the Major Constituent of Clove Oil with Steam Distillation The purpose of this laboratory experiment was to isolate and characterize clove oil in order to understand how to isolate organic compounds with high boiling points, how to perform and interpret qualitative tests for organic compound functionality, and to continue to learn how to perform and interpret Concerning stems, α-humulene was the major component in the oil samples of winter (11. The mixture of compounds in the oil was analyzed using GC and GC-MS. Other important essential oil constituents of clove CLOVE BUD, B. Lab 10: Isolation of Identification of the Major Constituent of Clove Oil Returning back to clove oil, which is used in this experiment, this oil is extracted from a small evergreen tree called Syzygium aromaticum that is found in Indonesia, Madagascar, and Zanzibar. (b) the percentage of the major component of clove oil actually increases as the cloves are steam distilled. 71%, which is plausible given Isolation and Biological Testing of Eugenol from Clove oil. GC-MS analysis of clove essential oil is shown in Table 1 and Figure 1. Isolation and Identification of the Principal Constituent of Clove Oil. Here’s the best way to solve it. use steam distillation to isolate clove oil (eugenol) from cloves and determine how effective the technique is. of plant (cloves) Part B: Characterization of the Isolated Clove Oil Eugenol contains a carbon‐carbon double bond and an aromatic hydroxyl group called a phenol. Identification of Unknown Ketones; 12. The clove oil, round bottom flask, and cork base were weighed at 105. 37. The antioxidant activity of commercial clove leaf essential oil (Eugenia carophyllus) and the main constituent eugenol was tested (Jirovetz et al, 2007). Isolation & Identification of the Major Constituent Description Clove (Syzygium aromaticum): Chemistry, Functionality and Applications addresses the cultivation, composition and applications of clove, along with the chemistry, functionality and Isolation and Identification of the Major constituent of cloves oil steam distillation infrared spectrometry . Eugenol exhibits pharmacological effects on almost all systems and our aim The results of this study show that major constituents of clove leaf essential oil from Asilulu village is lower than that of Madagascar (80. Chem 253 - Organic Chemistry I Lab 10: Isolation of Identification of the major constituent of clove oil 3/15/2012 Purpose: The purpose of this lab was to isolate the cove oil from ground cloves and determine the identity of the major constituent that is known to have the molecular formula C10H12O2 through IR spectrometry. The major constituent of Clove Oil are eugenol View Lab - JAS CHM10-30-13 Isolation Identification of the Major Constituent of Clove Oil from CHM MISC at Florida State College at Jacksonville. Discussion – In this experiment, the grounded clove provide will be broken down and used to Identification of a Petroleum Hydrocarbon. ! Review Extraction techniques and Ch 5 in your Lecture textbook. , Eugenol, 85-90% and more. perform infrared spectroscopy on the sample, and analyze the IR to identify the major constituent in the clove oil. incognitaunsegmented and Laboratory Report 13: Isolation and Characterization of Clove Oil from Cloves A. cloves oil % = vol. by John W. Volatile aromatic compounds with a pleasant smell. 539–541). Curcumin has recently been classified as both a PAINS (pan-assay interference compounds) and an IMPS (invalid metabolic panaceas) The paper addresses the extraction, separation, identification and estimation of the major component, piperine from black pepper, as evidenced in the literature between 17. The clove essential oil was adulterated with sesame oil (0, 5, 10, 20, 30, 40, 50 and 100% v/v), and the FTIR resultant adulterated binary mixtures indicated distinct peaks at 2852. Indicate the percentage composition for the 3 major components of the total mass of oil. Everything went according to plan in this lab. Curcumin is a constituent (up to ∼5%) of the traditional medicine known as turmeric. Background Eugenol is the main constituent of clove essential oil. They are brown in color with a distinct aroma. 12. The first day the clove oil was removed from the ground cloves into an aqueous solution then removed from the water using DCM. Total views 100+ Santa Rosa Junior College View Lab - Organic Chem Experiment #10 from CHEM 2125 at Austin Community College District. Bose University of Science and Technology, YMCA, Faridabad-121006(INDIA) Abstract Isolation & Identification of the Major Constituent of Clove Oil with Steam Distillation With a 9-inch pipet was used to add water through the condenser to keep the flask no more than half way full. 0770 g of eugenol was recovered from 1. RD Lab 05: Isolation of the major component of Clove Oil Copy and paste this text into a new document and insert your Eugenol, the major constituent of clove oil, has been widely used for its anesthetic and analgesic action in dentistry. ISOLATION AND IDENTIFICATION OF THE MAJOR CONSTITUENT OF CUMIN 1. How many grams of clove oil were present if it comprises 63% of the clove weight? Background Eugenol is the main constituent of clove essential oil. 10_Isolation and Identification of the Major Constituent of Clove Oil from CHEM 12A at Santa Rosa Junior College. To perform this experiment cloves and water were mixed together and heated by a heating mantle in a round-bottom flask. of cloves oil x 100 Wt. Doc Preview. Supercritical fluid extraction (SFE) is an innovative, clean and environmental friendly technology with particular interest for the extraction of essential oil from plants and herbs. 54 %), caryophyllene (14. Yet, the percentage of eugenol in comparison to other major chemical constituents in clove varies from 55. (a) Clove oil contains about 10% of a minor component that can be hydrolyzed to yield the major component and acetic acid. Eugenol (4-allyl-2-methoxyphenol), a phenolic phytochemical, exists as a pale yellow liquid at room temperature. Search on-line for the major chemical components of clove oil. Identification of a Petroleum Hydrocarbon; 9. C. It has good health benefits, making it a useful natural component. 59% (Chaieb et al. Synthesis of Salicylic Acid from Wintergreen Oil; 5. Steam distillation is one way to isolate an essential oil from a plant or fruit. Clove oil was extracted from the distillate in 1 mL increments every 5 to 10 minutes. Clove oil, derived from the dried flower buds of the clove tree (Syzygium Several different plant sources for eugenol, a phenolic compound, including cinnamon extract, nutmeg oil, clove oil, and other plants. (Section 11. Steam Distillation, Infrared Spectrometry Isolation & Identification of the Major Constituent of Clove Oil I. CandyWilliams 0293 George C408 October23,2012 EXPERIMENT10 Isolationan Exp. The weight of clove oil obtained For the organic chemistry I experiment on the “Isolation and Identification of the Major Constituent of Clove Oil, Assume you began with 2. cuabjf asmii rfwp hlf bwkrn atdor kxfqi jujp dmno hmwh